Fig. 2.

Validation of EpiVIA with clonal cells. (A) Chimeric and provirus fragments in HEK293 clone #1 identified by EpiVIA. (B) Genome browser view of reads and identified chimeric reads from HEK293 clone #1 mapped to the context of predefined integration site measured by LM-PCR. (C) Chimeric and provirus fragments in HEK293 clone #2 identified by EpiVIA. (D) Genome browser view of reads and identified chimeric reads from HEK293 clone #2 mapped to the context of predefined integration site measured by LM-PCR. (E) Genome browser view of reads and identified chimeric reads from two replicates of CAR⁺ and CAR⁻ CD8⁺ cells from a CLL patient at the predefined integration site measured by LM-PCR. (F) Chimeric and provirus fragments in CAR⁺ and CAR⁻ CD8⁺ T cells from a CLL patient identified by EpiVIA.