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. 2020 Feb 24;117(10):5319–5328. doi: 10.1073/pnas.1912864117

Fig. 1.

Fig. 1.

LARP1 is the primary regulator of known TOP mRNAs. (A) mTOR controls the translation of classical TOP mRNAs. RNA-seq and ribosome profiling libraries were prepared from WT HEK-293T cells treated with 250 nM Torin 1 for 2 h (n = 2). (A, Top) Translation efficiencies (RPF/RNA) of mRNAs in control or Torin 1-treated conditions. Classical TOP mRNAs indicated in red. Differentially translated mRNAs are indicated in blue (fold change translation efficiency >2, adjusted P < 0.01). (A, Bottom) Changes in translation efficiencies between Torin 1 and control conditions for classical TOP and other mRNAs. (B) LARP1 is required for mTOR regulation of classical TOP mRNA translation. sgLARP1 cells were treated and analyzed as in A (n = 2). (C) Profiles of ribosome protected fragments (RPFs), for example, TOP (RPL3) and non-TOP (ACTB) mRNAs from A and B. (D) LARP1 regulation of classical TOP mRNAs. Changes in translation efficiency of indicated mRNAs in WT or sgLARP1 HEK-293T from A and B. Significance by Wald test (Benjamini–Hochberg adjustment; *P < 0.01, **P < 10−10, ***P < 10−50).