Fig. 6.

Knockdown of RBM14 reduces high-fidelity cNHEJ and increases mutagenic NHEJ. (A) Knockdown of RBM14 increases error-prone, Mut-NHEJ. Knockdown of DNA-PKcs was performed in parallel. DNA DSB repair was measured in HEK293T cells containing a single copy of an integrated reporter that undergoes Cas9-mediated DNA DSBs and subsequent repair by NHEJ. Bar graphs are represented as the mean values of three independent experiments ± SD; *P < 0.05, **P < 0.01, ***P < 0.001. (B) Western blots of protein lysates from HEK293T cells containing the reporter construct and transfected with the indicated siRNAs. The values indicated under the blot are the mean fold protein expression relative to control taken as 1 after normalization by β-actin (ImageJ quantification).