Fig. 2.

Involvement of Ms’myo in photoperiodic regulation of nymphal development in the cricket Modicogryllus siamensis. (A and B) Expression patterns of Ms’myo (A) and Ms’jhamt (B) mRNAs during nymphal development under long-day (LD, 16L:8D, circles) and short-day (SD, 12L:12D, squares) conditions at 25 °C (blue) or 30 °C (orange). Ms’myo mRNA levels were significantly higher under LD than SD for the last three instar nymphs. Ms’jhamt levels tended to be higher under SD throughout the tested period. Values were an average of four samples, including three heads each, and error bars indicate SEM. a and b indicate a significant difference between LD and SD at 25 °C and 30 °C, respectively. (C–F) Effects of Ms’myo nymphal RNAi (Ms’myo^nRNAi) on adult emergence under LD (C) and SD (D), molt numbers (E), and body weights (F) at 25 °C. Gray and green symbols in C, D, and F indicate DsRed2^nRNAi control and Ms’myo^nRNAi crickets, respectively. N and numbers in parenthesis indicate the number of animals used. Numbers in E indicate the number of molts. Ms’myo^nRNAi significantly delayed adult emergence (U test, P < 0.001) and increased molt numbers compared with that of DsRed2^nRNAi crickets (U test, P < 0.001). Body weights were significantly increased in Ms’myo^nRNAi-treated crickets under LD (Tukey test, P < 0.05). Values with different letters significantly differ from each other.