Table 1.

Study Exclusion Criteria

Dysmenorrhoea on Day 1–2 reported as 4–6 on a 10-point scale

Menstrual cycle length less than 26 or more than 30 days

Irregular menstrual cycles

Previous pregnancy

Use of reproductive hormones (apart from OC)

Use of levonorgestrel-releasing intrauterine device

Use of thyroxine, insulin or corticosteroids

BMI less than 16 or more than 30

Inflammatory process, surgical procedure or infection in previous 4 weeks

Renal, hepatic, cardiac or auto-immune disease

Use of immunosuppressant medications

Use of medications affecting TLR responsiveness, including amitriptyline and minocycline

Use of analgesics including anti–inflammatory drugs, opioids or paracetamol for 5 drug half-lives prior to testing

Use of alcohol for 24 hrs prior to testing

Use of opioids or marijuana for 30 days prior to testing

Inability to read or comprehend written information provided