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. 2020 Feb 6;98(3):skaa044. doi: 10.1093/jas/skaa044

Figure 1.

Figure 1.

Respirometry protocol and example trace using permeabilized fibers from Angus or Brahman Longissimus lumborum muscle at 1-h postmortem. Oxygen flux per mass (ρmol/s/mg tissue; red line), and oxygen concentration (nmol/mL; blue line). The following SUIT protocol was performed: 1) malate, glutamate, pyruvate (2, 10, and 5 Mm, respectively; LEAK respiration, L); 2) ADP (2.5 mM; activated respiration, PCI); 3) succinate (10 mM; activated respiration, PCI+II), 4) cytochrome c (10 μM; quality control; see text for details); 5) rotenone (0.5 μM; activated respiration after inhibition of complex I, PCII); 6) oligomycin (5 nM; LEAK respiration induced by oligomycin-induced inhibition of ATP synthase; LO); 7) carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone (FCCP, 0.05 μM steps; maximum succinate-supported electron transport capacity, ECII); 8) antimycin A (2.5 μM; residual oxygen consumption, ROX).