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. Author manuscript; available in PMC: 2020 Jun 11.
Published in final edited form as: Oncogene. 2019 Dec 11;39(11):2275–2289. doi: 10.1038/s41388-019-1141-7

Figure 5. CCL2 shRNA knockdown inhibits migration but not proliferation of mammary carcinoma cells.

Figure 5.

PyVmT cells stably expressing control (Con-sh) or two different CCL2 shRNAs (CCL2-sh1, CCL2-sh3) were analyzed for A. CCL2 expression by ELISA, B. PCNA expression and C-D. wound closure in Con-si (C) or CCR2-si transfected cells (D). PCNA staining was quantified by Image J. Expression was normalized to DAPI, arbitrary units are shown. Statistical significance was determined by p<0.05. *p<0.05, **p<0.01, ns=not significant. Mean±SEM are shown.