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. Author manuscript; available in PMC: 2021 Apr 1.
Published in final edited form as: J Orthop Res. 2019 Nov 27;38(4):731–739. doi: 10.1002/jor.24525

Figure 4: The effect of EVs isolated from the medium of vehicle-treated articular chondrocytes on catabolic and anabolic marker mRNA levels of articular chondrocytes.

Figure 4:

mRNA levels of catabolic markers Cox-2, IL-6, iNOS, MMP-13 and articular cartilage markers aggrecan and type II collagen (α1(II)) in IL-1β-treated human articular chondrocytes cultured in the absence or presence of EVs isolated from vehicle-treated (EVs) human articular chondrocytes. mRNA levels were determined by real time PCR analysis using SYBR Green and normalized to the levels of 18S RNA. Values are expressed as relative units with the mRNA levels of IL-1β-treated human articular chondrocytes set as 1. Data were obtained from four different experiments and are expressed as mean ± SD. *p < 0.01 vs. IL-1β treated cells.