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. Author manuscript; available in PMC: 2021 Apr 1.
Published in final edited form as: J Orthop Res. 2019 Nov 27;38(4):731–739. doi: 10.1002/jor.24525

Figure 5: The effect of conditioned medium from the medium of vehicle-treated or IL-1β-treated articular chondrocytes containing EVs or after complete removal of EVs on chondrogenic differentiation of C3H/10T1/2 cells.

Figure 5:

mRNA levels of aggrecan, type II collagen (α1(II)), Sox9, and type I collagen (α1(I)) during chondrogenic differentiation of C3H/10T1/2 cells cultured in micromasses in the absence or presence of BMP-2 (BMP) and conditioned medium containing EVs from vehicle-treated (CM) or IL-1β-treated (IL-1CM) mouse articular chondrocytes or conditioned medium from vehicle-treated (CM spin) or IL-1β-treated (IL-1CM spin) mouse articular chondrocytes, from which EVs were completely removed by ultracentrifugation. mRNA levels were determined by real time PCR analysis using SYBR Green and are expressed as relative units with the mRNA levels of untreated micromasses set as 1. Data were obtained from four different experiments and are expressed as mean ± SD. *p < 0.01; **p < 0.05.