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. Author manuscript; available in PMC: 2021 Mar 13.
Published in final edited form as: ACS Infect Dis. 2020 Jan 24;6(3):393–405. doi: 10.1021/acsinfecdis.9b00354

Scheme 2. The proposed model of oxidase activity of C-terminus modified TGR.

Scheme 2.

The reaction scheme was drawn following the experimental results reported in this work and previous studies.21, 24 The nucleophilic Sec residue of reduced TGR could be covalently modified by electrophilic aromatic inhibitors (A). The modified C-terminus moves to FAD redox center of the other subunit (B). The electrophilic aromatic ring of bonded inhibitor accepts a single electron from the thiol of proximal C154 and then transfers it to oxygen molecule to produce superoxide (C – E). EWG = electron withdrawing group.