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U597C and ΔCUG (each at 500 nM) were incubated with NADPH (100 μM) and compounds for 30 minutes. Samples were then desalted. NADPH consumption, superoxide production using pyrogallol red, and H2O2 production using Amplex Red and horseradish peroxidase, were determined. Addition of 10 units of SOD abolished oxidation of pyrogallol red and addition of 20 units of catalase inhibited oxidation of Amplex Red (not shown).
