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. 2020 Jan 17;98(3):skaa003. doi: 10.1093/jas/skaa003

Table 1.

Diet composition (as-fed basis)1

Item Control Cu CTC Cu + CTC
Ingredients, %
 Corn 56.05 55.97 55.62 55.54
 Soybean meal, 47% crude protein 24.76 24.77 24.79 24.80
 Dried whey 10.00 10.00 10.00 10.00
 Enzyme-treated soybean meal2 5.00 5.00 5.00 5.00
 Monocalcium phosphate, 21.5% phosphorus 1.20 1.20 1.20 1.20
 Calcium carbonate 1.05 1.05 1.05 1.05
 Sodium chloride 0.60 0.60 0.60 0.60
 L-Lysine HCl 0.45 0.45 0.45 0.45
 DL-Methionine 0.20 0.20 0.20 0.20
 L-Threonine 0.20 0.20 0.20 0.20
 L-Tryptophan 0.02 0.02 0.02 0.02
 L-Valine 0.05 0.05 0.05 0.05
 Trace mineral premix3 0.15 0.15 0.15 0.15
 Vitamin premix4 0.25 0.25 0.25 0.25
 Phytase5 0.02 0.02 0.02 0.02
 Copper sulfate6 0.08 0.08
 Chlortetracycline7 0.40 0.40
 Total 100 100 100 100
Calculated analysis
 SID8 amino acids, %
  Lysine 1.35 1.35 1.35 1.35
   Isoleucine:lysine 57 57 57 57
   Leucine:lysine 115 115 115 115
   Methionine:lysine 36 36 35 35
   Methionine and cysteine:lysine 57 57 57 57
   Threonine:lysine 64 64 64 64
   Tryptophan:lysine 18.5 18.5 18.5 18.5
   Valine:lysine 65 65 65 65
  NE, kcal/kg 2436 2434 2425 2423
  Crude protein, % 21.1 21.1 21.1 21.1
  Total calcium, % 0.79 0.79 0.79 0.79
  STTD9 phosphorus, % 0.53 0.53 0.53 0.53
Analyzed composition
  Dry matter, % 88.2 88.1 88.1 88.2
  Crude protein, % 20.6 20.7 20.9 21.1
  Calcium, % 0.85 0.91 0.93 0.91
  Phosphorus, % 0.63 0.65 0.69 0.66
  Copper, mg/kg 24 202 31 194
  Chlortetracycline, mg/kg ND10 0.06 539 309

1Diets were fed in meal form from 7.4 to 19.7 kg BW. Copper (Cu) sulfate and chlortetracycline (CTC) were included in the diet at the expense of corn.

2HP 300 (Hamlet Protein, Inc., Findlay, OH).

3Provided per kg of premix: 73 g Zn from Zn sulfate; 73 g Fe from iron sulfate; 22 g Mn from manganese oxide; 11 g Cu from copper sulfate; 0.2 g I from calcium iodate; 0.2 g Se from sodium selenite.

4Provided per kg of premix: 3,527,399 IU vitamin A; 881,850 IU vitamin D; 17,637 IU vitamin E; 1,764 mg vitamin K; 15.4 mg vitamin B12; 33,069 mg niacin; 11,023 mg pantothenic acid; 3,307 mg riboflavin.

5Ronozyme Hiphos 2700 (DSM Nutritional Products, Inc., Basel, Switzerland) provided 476 phytase units per kg of feed.

6Copper sulfate provided 200 mg/kg of Cu.

7Aureomycin 50 (Zoetis Services, LLC., Parsippany, NJ) provided 440 mg/kg CTC.

8SID, standardized ileal digestible.

9STTD, standardized total tract digestible.

10ND, not detected above the detection limit of 0.05 mg/kg.