Table 5.
Effect of time and treatment on the relative percentage abundance of genera in the porcine microbial community as determined by 16S rRNA gene sequencing
| Top 20 genera | Treatment1 | ||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Control | EO | FA | SC | EO + SC | FA + SC | EO + FA | |||||||||
| 0 h | 6 h | 24 h | 6 h | 24 h | 6 h | 24 h | 6 h | 24 h | 6 h | 24 h | 6 h | 24 h | 6 h | 24 h | |
| Lactobacillus | 17.31 | 4.90 | 2.64 | 18.41 | 19.52 | 63.48 | 55.08 | 11.08 | 4.52 | 16.51 | 22.61 | 34.28 | 46.47 | 18.82 | 23.41 |
| Ruminococcaceae (family) | 28.84 | 1.71 | 9.62 | 26.64 | 24.42 | 11.89 | 14.14 | 3.51 | 10.90 | 26.43 | 25.69 | 22.26 | 16.47 | 25.86 | 25.88 |
| Streptococcus | 5.75 | 3.77 | 1.93 | 3.93 | 4.21 | 2.18 | 2.04 | 72.89 | 33.33 | 3.53 | 4.37 | 3.32 | 2.79 | 4.57 | 4.13 |
| Enterobacteriaceae (family) | 8.75 | 34.18 | 23.83 | 2.36 | 3.57 | 1.67 | 2.49 | 1.62 | 22.34 | 2.42 | 3.29 | 2.22 | 1.58 | 3.35 | 2.94 |
| Clostridiaceae (family) | 2.72 | 42.50 | 36.73 | 2.24 | 2.29 | 0.96 | 1.11 | 1.27 | 1.62 | 2.25 | 2.13 | 1.74 | 1.60 | 2.72 | 2.56 |
| Clostridiales (order) | 9.36 | 0.55 | 0.94 | 8.67 | 9.52 | 3.91 | 4.77 | 1.27 | 0.59 | 8.87 | 9.83 | 7.35 | 5.67 | 8.74 | 9.50 |
| Lachnospiraceae (family) | 3.35 | 0.45 | 2.35 | 5.06 | 5.16 | 1.85 | 2.26 | 0.58 | 1.28 | 4.96 | 4.97 | 3.66 | 2.76 | 5.61 | 4.89 |
| Methanobrevibacter | 0.52 | 0.23 | 0.14 | 5.53 | 5.34 | 2.91 | 6.02 | 0.44 | 0.03 | 6.70 | 1.25 | 5.35 | 6.87 | 0.66 | 0.29 |
| Bacteroidales (order) | 4.20 | 0.19 | 0.56 | 3.29 | 3.02 | 1.38 | 1.47 | 0.55 | 1.24 | 3.33 | 2.80 | 1.98 | 1.75 | 3.54 | 2.76 |
| Clostridium | 1.27 | 8.73 | 8.16 | 0.96 | 0.97 | 0.41 | 0.48 | 0.40 | 0.52 | 1.01 | 0.95 | 0.71 | 0.63 | 0.95 | 1.05 |
| SMB53 | 0.84 | 0.09 | 2.47 | 1.09 | 1.16 | 0.50 | 0.47 | 0.23 | 7.63 | 1.06 | 1.20 | 1.04 | 0.73 | 1.47 | 1.52 |
| S24-7 (family) | 2.15 | 0.09 | 0.13 | 2.61 | 1.94 | 1.02 | 1.06 | 0.33 | 0.09 | 2.65 | 1.82 | 1.50 | 1.14 | 2.83 | 1.94 |
| Ruminococcus | 1.95 | 0.32 | 3.27 | 2.05 | 2.09 | 0.93 | 1.00 | 0.44 | 0.46 | 2.09 | 2.22 | 1.79 | 1.37 | 2.16 | 2.24 |
| Coprococcus | 1.03 | 0.10 | 0.19 | 2.20 | 2.39 | 0.89 | 1.13 | 0.15 | 0.08 | 2.12 | 2.65 | 1.86 | 1.46 | 2.15 | 2.67 |
| Oscillospira | 1.69 | 0.11 | 1.88 | 1.71 | 1.85 | 0.80 | 1.03 | 0.21 | 0.83 | 1.75 | 2.15 | 1.32 | 1.16 | 1.55 | 2.02 |
| Treponema | 0.98 | 0.08 | 0.06 | 2.02 | 1.50 | 0.70 | 0.59 | 0.29 | 0.10 | 3.36 | 1.16 | 1.51 | 1.59 | 2.78 | 1.20 |
| Christensenellaceae family) | 1.27 | 0.12 | 0.11 | 1.26 | 1.26 | 0.58 | 0.70 | 0.20 | 0.07 | 1.29 | 1.32 | 0.96 | 0.76 | 0.95 | 1.21 |
| Dorea | 0.91 | 0.07 | 0.19 | 1.09 | 1.21 | 0.58 | 0.59 | 0.15 | 0.06 | 1.09 | 1.31 | 1.01 | 0.67 | 1.35 | 1.33 |
| CF231 | 1.21 | 0.02 | 0.11 | 1.10 | 1.18 | 0.39 | 0.49 | 0.06 | 0.02 | 1.02 | 1.21 | 0.70 | 0.64 | 1.03 | 1.08 |
1Treatments designations: Control, no additives; 1.5% vol/vol solution of EO mixture; 2.5 mg/mL of FA; 10 Mm of SC, as well as mixtures of EO + SC, EO + FA, and FA + SC at each of the aforementioned concentrations.