. Author manuscript; available in PMC: 2021 Jan 23.

Published in final edited form as: Cell. 2020 Jan 16;180(2):373–386.e15. doi: 10.1016/j.cell.2019.12.029

Table 1.

Top 100 Cell-Surface Enriched Proteins in Developing PNs

1	Gfrl		frazzled^*		Dscam3^*		Semaphorin 2a^*^,g,i
	Tenectin^*		DIP-δ^*^,f		mind-meld		CG44837
	Wrapper^*		dpr14^*		friend of echinoid^*		DIP-β^*
	N-cadherin^*^,a,b		Semaphorin 2b^*^,g,h,i		Dscam4^*		Fdh
5	CG34118 (Gfrl)	30	sidestep III^*	55	frizzled 2^*^,j	80	Agpat3
	CG31998^#		CG16791		CG3036^#		CG1607
	Neurotactin^*		turtle^*		distracted^*		CD98hc
	Dscam1^*^,c,d		sidestep VII^*		Alk^*		ReepB
	Toll-7^*^,e		GILT1^#		Lar^*		farjavit
10	beaten path IV^*	35	beaten path IIIc^*	60	santa-maria	85	Lachesin
	Fasciclin 3^*		sticks and stones^*		Neuroglian^*^,k,l		Tol l-6^*^,e
	Ptp10D^*		Agpat1		Orct		omega
	kekkon 1^*		Toll-2^*^,e		CG34353^#		Neurexin IV^*
	Oct-TyrR		myospheroid^*		Fasciclin 2^*		smoke alarm^#
15	Connectin^*	40	dpr21^*	65	Plexin A^*^,m	90	klingon^*
	hibris^*		CG17839^#		Semaphorin 1 a^*^,m,n,o		CG5789
	CG1504		Contactin^*		Neuroligin 4^*		Teneurin-m^*^,p,q
	sidestep^*		Transferrin 2		kekkon 5		starry night^*
	Dscam2^*		Cirl		Teneurin-a^*^,p,q		tincar^*
20	dally-like^*	45	CG7166^#	70	beaten path VI^*	95	Plexin B^*^,g,h,s
	CG43737^#		kugelei^#		roundabout 1^*^,r		DIP-γ^*^,f
	Dystroglycan^*		GluRIA		DIP-α^*		crumbs^*
	Neprilysin 3^#		Chitinase 2^#		CG42402		CG42346
	Multiplexin^*		prominin-like^*		dpr8^*		CG34449
25	Megf8^#	50	Tsp42Ej	75	roundabout 3^*^,r	100	Mgstl

Proteins are ranked by the 127C:130C TMT ratio (Figure 2B).

denote proteins with known function in neural development, in particular circuit wiring.

denote newly identified wiring molecules in this study (Figure 4 and Table 2). Gfrl and CG34118 are listed as two separate entries in the UniProt Drosophila reference proteome, but are annotated as one gene (FBgn0262869) in the FlyBase.

References for previously identified wiring molecules in the fly olfactory circuit are as follows:

(Hummel and Zipursky, 2004);

(Zhu and Luo, 2004);

(Hummel et al., 2003);

(Zhu et al., 2006);

(Ward et al., 2015);

(Barish et al., 2018);

(Joo et al., 2013);

(Li et al., 2018);

ⁱ

(Sweeney et al., 2011);

(Singh et al., 2010);