Table 2.
RNAi Screen in Ventromedial (VM) PNs and ORNs: Genes, Molecular Features, Phenotypes, and Penetrances
| Gene | Human Ortholog | Molecular Feature | RNAi Phenotype | % Phenotypic Penetrance (n) |
|---|---|---|---|---|
| CG7166 | – | Immunoglobulin and fibronectin domains | ORN trajectory error and dorsal mistargeting | 74.1 (58), 38.9 (54) |
| CG7466/Megf8 | MEGF8 | Epidermal growth factor domain | ORN ventral mistargeting | 62.0 (50), 40.6 (64) |
| CG7749/kug | FAT3 | Cadherin repeat | PN ventral mistargeting | 98.0 (52), 62.0 (50) |
| CG17839 | – | Immunoglobulin and fibronectin domains | PN lateral mistargeting | 48.1 (52), 37.5 (32) |
| CG33087/LRP1 | LRP1 | Low-density lipoprotein receptor | PN and ORN local mistargeting | 41.7 (48), 36.5 (52), 65.4 (52), 39.3 (28) |
| CG34353 | LSAMP | Immunoglobulin and fibronectin domains | ORN posterior mistargeting | 22.9 (48), 21.2 (52) |
| CG2054/Cht2 | CHIA | Chitinase | ORN medial mistargeting | 83.3 (54), 21.4 (42) |
| CG3036 | – | Anion transporter | ORN posterior mistargeting | 34.0 (50), 40.3 (62) |
| CG3921/bark | – | Scavenger receptor | PN and ORN local mistargeting | 73.1 (52), 23.9 (46) |
| CG4645 | YIPF1 | Yip domain | ORN medial mistargeting | 50.0 (50), 98.1 (54)* |
| CG6113/Lip4 | LIPM | Lipase | PN ventral mistargeting | 92.0 (50), 30.0 (30) |
| CG6821/Lsp1Y | – | Hemocyanin domain | Global disruption | 100.0 (24), 56.0 (50) |
| CG8460 | CHID1 | Chitinase | ORN dorsal mistargeting | 69.6 (56) |
| CG9565/Nep3 | ECE1 | Neprilysin peptidase | ORN ventral mistargeting | 68.5 (54), 60.7 (56) |
| CG9796/GILT1 | IFI30 | Thiol reductase | PN and ORN local mistargeting | 83.3 (36), 87.0 (54) |
| CG14234 | TMEM198 | – | PN and ORN local mistargeting | 68.9 (58), 100.0 (58)* |
| CG14446/dtn | TMEM132E | – | ORN dorsal mistargeting | 70.4 (54), 51.9 (52) |
| CG31998 | – | – | ORN dorsal mistargeting | 70.0 (60), 50.0 (58) |
| CG34380/smal | DDR2 | Coagulation factor | PN random mistargeting | 39.6 (48), 26.9 (52) |
| CG43737 | – | – | ORN dorsal and PN random mistargeting | 28.9 (52), 83.3 (54) |
Human orthologs were searched by the FlyBase Homologs Search tool. Only the orthologs consistently identified by four or more databases are listed. Molecular features were searched through FlyBase and UniProt. The top 6 proteins in the table belong to families of classic wiring molecules based on their structural domains; the bottom 14 proteins come from molecular families not previously linked to neural development. Phenotypic penetrance of each RNAi is listed with the number of antennal lobes examined in parentheses. Antennal lobe image of each RNAi is included in Figure S5.
represent two cases where pan-neuronal RNAi was lethal and PN-GAL4 was used instead to drive RNAi expression.