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HPLC analysis parameters
| Parameter | Mycotoxins | ||||
|---|---|---|---|---|---|
| AFB1 | DON | FUM | T-2 | ZEN | |
| Column heating | – | 30 | – | – | – |
| Mobile phase | Water/acetonitrile/methanol (60:30:10) | Water/acetonitrile (90:10) | Gradient methanol/water (70:30 and 80:20) | Methanol/water (60:40) | Methanol/water (70:30) |
| Fluorescent detector λ (nm) (excitation and emission) | 360 and 420 | – | 490 and 450 | 381 and 470 | 280 and 460 |
| UV detector λ (nm) | – | 218 | – | – | – |
| Flow (ml/min) | 1 | 1 | 1 | 1 | 1 |
