Table 2.
Major chemical compounds of CRP identified by LC/MS-IT-TOF.
| Peak no. | RT (min) | [M + H]+ (m/z) | Molecular formula | Fragment ions (m/z) | Identified compounds |
|---|---|---|---|---|---|
| 2 | 14.746 | 581.1854 | C27H32O14 | 563.1743, 545.1634, 401.1057, 339.0855, 315.0814, 273.0756, 263.0544, 245.0433, 231.0385, 219.0280, 195.0291, 179.0287, 153.0179, 135.0564 | Naringin |
| 3a | 14.995 | 611.1956 | C28H34O15 | 580.6021, 465.1423, 449.1420, 431.1322, 413.1220, 345.0960, 303.0855, 281.0644, 263.0533, 219.0288, 195.0278, 177.0539, 153.0179 | Hesperidin |
| 4 | 16.162 | 611.1957 | C28H34O15 | 596.2038, 575.1750, 465.1420, 449.2690, 431.1318, 413.1227, 369.0966, 345.0954, 303.0864, 281.0647, 263.0539, 219.0288, 195.0280, 177.0540, 153.0179 | Neohesperidin |
| 5 | 18.506 | 595.2011 | C28H34O14 | 449.1152, 433.1460, 397.1286, 379.1211, 365.0712, 287.0888, 256.0431, 224.1293, 195.0290, 183.0582, 165.0598 | Poncirin |
| 6 | 26.814 | 373.1273 | C20H20O7 | 358.1033, 343.0800, 327.0501, 299.0504, 285.0613, 189.0301, 165.0171, 151.0752 | Isosinensetin |
| 7 | 28.119 | 389.1224 | C20H20O8 | 374.0996, 360.0797, 359.0761, 341.0662, 331.0804, 328.0944, 313.0592, 211.0154, 193.0088 | Monohydroxy-pentamethoxyflavone |
| 8 | 31.411 | 373.1265 | C20H20O7 | 357.0960, 343.0837, 339.0865, 329.1018, 327.0533, 313.0688, 312.1002, 297.0766, 269.0820, 181.0395 | Sinensetin |
| 9a | 36.272 | 403.1386 | C21H22O8 | 388.1121, 373.0921, 355.0809, 345.0944, 342.1099, 327.0657, 313.0708, 258.0526, 183.0296, 165.0519 | Nobiletin |
| 10 | 37.217 | 343.1140 | C19H18O6 | 327.0402, 313.0709, 309.0764, 299.0914, 282.0887, 267.0650, 153.0186, 135.0642 | Tetramethyl-O-scutellarin |
| 11a | 39.931 | 433.1510 | C22H24O9 | 418.1239, 417.1167, 403.1003, 385.0903, 373.0529, 345.0600, 317.0655, 183.0288, 165.0188 | 3,5,6,7,8,3′,4′-Heptamethoxyflavone |
| 12a | 41.968 | 373.1281 | C20H20O7 | 358.1050, 343.0812, 328.0579, 325.0725, 312.0993, 297.0761, 283.0589, 211.0256, 183.0292, 135.0442 | Tangeretin |
| 13 | 42.637 | 375.1458 | C19H18O8 | 345.0606, 327.0507, 299.0558, 227.0550, 211.0613, 196.0361, 183.0287, 167.0343 | Dihydroxy-tetramethoxyflavone |
| 14a | 44.908 | 389.1227 | C20H20O8 | 374.0996, 359.0761, 356.0886,343.0440, 341.0662, 331.0804, 328.0944, 327.0862, 298.0473, 197.0092, 165.0758, 151.0520 | 5-Hydroxy-6,7,8,3′,4′-pentamethoxyflavone |
| 15 | 45.577 | 405.1564 | C20H20O9 | 375.1424, 345.1332, 329.1027, 241.0711, 226.0477, 211.0238, 165.0187 | Dihydroxy-pentamethoxyflavone |
| 16 | 46.073 | 419.1326 | C21H22O9 | 404.1032, 389.0983, 371.0802, 357.0587, 163.0385 | Monohydroxy-hexamethoxyflavone |
| 17 | 47.053 | 359.1138 | C19H18O7 | 344.0858, 329.0659, 326.1001, 311.0568, 298.0835, 210.1810 | 5-Hydroxy-6,7,8,4′-tetramethoxyflavone |
| 18 | 48.439 | 375.1424 | C19H18O8 | 345.0898, 241.0654, 226.0423, 211.0211, 183.0290 | Dihydroxy-tetramethoxyflavone |
aConfirmed in comparison with standard compounds.