Figure 3.

TA induces cell cycle arrest in NCCIT cells. (A) Flow cytometry analysis showing sub-G1 arrest after treatment with 25 µM and 50 µM TA for 24 h and 48 h in NCCIT cells. (B) Graphical representation of the cell distribution in the sub-G1 phase. Experiments were repeated three times, and mean values are presented in the final graph. (C) Western blotting analysis of cell cycle markers after treatment with TA for 48 h. (D) The representative expressions of the p21, p27, cyclin D1, cyclin E, and CDK4 proteins were determined by densitometry and normalized to β-actin. Controls are set to 100. ** p < 0.01 and *** p < 0.001 (t-test).