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. 2020 Feb 11;9(2):414. doi: 10.3390/cells9020414

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Subchronic effects of VPA (1000 and 3000 μM) (A) and CLZ (3, 10, and 30 μM) (B) on Cx43 expression in cytosol (blue columns) and plasma membrane (green columns) of astrocytes. Acute effects of CLZ (3, 10, and 30 μM) on Cx43 expression in cytosol and plasma membrane of subchronically treated astrocytes with therapeutic-relevant concentration of VPA (1000 μM) (C). Ordinate: mean ± SD (n = 6) of relative protein level of Cx43. Abscissa: concentration of VPA and CLZ (μM). * p < 0.05 and ** p < 0.01 vs. agent free by MANOVA with Tukey’s post hoc test. Panels 6(D) and 6(E) pseudo-gel images using Simple Western results using anti-GAPDH and anti-Cx43 antibody for blotting of cytosol and plasma membrane fractions, respectively.