Figure 5.

Changes in the expression of signaling molecules during senescence of pEOCs determined using immunoblotting. (a) Samples corresponding to 1 × 10⁴ (AKT, AP-1, ERK1/2, JNK, NF-κB, p38 MAPK, STAT3), 4 × 10⁴ (FOXO4, JAK3), and 5 × 10⁴ (β-actin) cells were subjected to SDS–PAGE to eliminate the risk of incorrect results due to senescence-associated cell hypertrophy and related differences in protein content between young and senescent cells. (b) Densitometric analysis of bands corresponding to young and senescent cells. Results are based on five to six independent experiments using pEOCs obtained from different patients. Results are expressed as mean ± SEM. * p < 0.05; ** p < 0.01 vs. early-passage cells.