Table 1.

Flow cytometry staining.

Analysis	Staining Solution	Manufacturer
ROS	DHR123 (Dihydrorhodamine 123), final concentration 200 nM	Sigma-Aldrich, St. Louis, MO, USA
RNS	DAF-FM (Diaminofluorescein-FM) diacetate, final concentration 2 mM	Sigma-Aldrich, St. Louis, MO, USA
Lipid peroxidation	BODIPY 581/591 C11, final concentration 1 mM	Sigma-Aldrich, St. Louis, MO, USA
Superoxide anion	MitoSox Red mitochondrial superoxide indicator, final concentration 5 µM	Sigma-Aldrich, St. Louis, MO, USA
Externalization of phosphatidylserine	Annexin V-FITC, 1:100 Propidium iodide, final concentration 25 µg/mL	BD Biosciences Pharmingen, San Diego, CA, USA
Caspase-3 activation	Cleaved caspase-3-PE, 1:200	BD Biosciences Pharmingen, San Diego, CA, USA
Cytochrome c release	Cytochrome c antibody (6H2) FITC conjugate, 1:200	Invitrogen, Carlsbad, CA, USA
Smac/DIABLO release	Smac/DIABLO rabbit mAb, 1:200	Cell Signaling Technology, Danvers, MA, USA
	Goat anti-rabbit IgG (H + L) secondary antibody, Alexa Fluor 488, 1:500	Thermo Scientific, Rockford, IL, USA
Mitochondrial membrane potential	TMRE (tetramethylrhodamine ethyl ester perchlorate), final concentration 0.1 µM	Sigma-Aldrich, St. Louis, MO, USA
Protein analysis	Phospho-Bcl-2 (Ser70) rabbit mAb Alexa Fluor 488 conjugate, 1:200 Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (E10) mouse mAb, 1:2000 Phospho-SAPK/JNK (Thr183/Tyr185) (G9) mouse mAb (PE conjugate), 1:200 Phospho-p38 MAPK (Thr180/Tyr182) (3D7) rabbit mAb PE conjugate, 1:200	Cell Signaling Technology, Danvers, MA, USA
	Goat anti-rabbit IgG (H+L) secondary antibody, Alexa Fluor 488, 1:500	Thermo Scientific, Rockford, IL, USA
DNA damage	Anti-pATM, PE conjugated antibody, 1:200 Anti-pHistone H2A.X, PerCP conjugated antibody, 1:200 Anti-pSMC1, Alexa Fluor 488 Antibody, 1:200	Millipore Corporation, Temecula, CA, USA
	Anti-oxoguanine 8 antibody	Abcam, Cambridge, United Kingdom
	Goat anti-mouse IgG (H + L) secondary antibody, Alexa Fluor 488	Thermo Scientific, Rockford, IL, USA