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. 2020 Feb 6;9(2):377. doi: 10.3390/cells9020377

Figure 5.

Figure 5

Effect of silk peptide on proteins involved in glucose uptake and mitochondrial metabolism in muscle from high-fat diet-fed mice. (A) Expression of proteins involved in glucose uptake (p-AMPK, AMPK, p-IRS, IRS, and GLUT4) was determined by western blotting. (B) Expression of proteins involved in mitochondrial biogenesis (PGC1α, NRF1, and UCP3) was determined by western blotting. (C) Immunofluorescence images of muscles were captured at 400× magnification. Muscles were fixed with methanol, and then anti-GLUT4 or anti-UCP3 antibodies and DAPI were applied. Data were analyzed using one-way ANOVA and Duncan’s test. Values with different letters are significantly different; p < 0.05 (a > b > c).