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. 2020 Feb 4;9(2):363. doi: 10.3390/cells9020363

Figure 1.

Figure 1

Features of the RNAi transformants. (a) PCR screening based on the amplification of the hygromycin resistance gene; (b) mycelial dry weight from wild-type P. italicum and the RNAi transformants; (c) spore number from wild-type P. italicum and the RNAi transformants; (d) qPCR expression analysis of the Dicer-like genes in the RNAi transformants and wild-type strain after culture in PDB (potato dextrose agar) for 5 days. CK (wild-type), BV (blank vector transformant), DCL1RNAi (Dicer-like1 gene transformant) and DCL2RNAi (Dicer-like 2 gene transformant), and BV1 and BV2 mean the relative expression level of Dicer-like 1 and Dicer-like 2 in the blank vector transformant, respectively. Each bar represents a mean ± standard error (n = 3 or 4), and the asterisk indicates a significant difference (***, p < 0.001).