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qRT-PCR expression analysis of the Dicer1-like gene (a) and Dicer2-like gene (b) in P. italicum after inoculation on citrus fruit epidermis for 3, 4, and 5 days. CK (wild-type), BV (blank vector transformant), DCL1RNAi (Dicer-like 1 gene transformant), and DCL2RNAi (Dicer-like 2 gene transformant). Each bar represents a mean ± standard error (n = 3), and the asterisk indicates a significant difference (**, p < 0.01, ***, p < 0.001), no significance (ns).
