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. 2020 Feb 18;12(2):468. doi: 10.3390/cancers12020468

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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FUT8 overexpression promotes DNA replication. Differentially expressed proteins (1.5-fold upregulated proteins) between LNCaP-FUT8/LNCaP-Ctr that were identified in our global proteomics analysis were subjected to the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis. Several proteins including the subunits of DNA polymerase alpha primase complex, DNA polymerase delta and epsilon along with minichromosome maintenance (MCM) and proliferation cell nuclear antigen (PCNA) were identified in LNCaP-FUT8 cells compared to the wildtype control (A). The same 1.5-fold upregulated proteomics data were analyzed for cell cycle analysis showing overexpression of many S phase and G2/M cell phase cycle proteins (B). Bubble blot showing the expression of PCNA and CDK2 expression correlation between the two LNCaP-FUT8 and LNCaP Ctr cell lines (C). Western Blot analysis showing PCNA and CDK2 expression between the LNCaP Ctr and LNCaP-FUT8 cell lysate. Actin was included that accounted for the amount of loading across lanes (D).