Table 1.

Sequences of sgRNAs and sequencing primers used for knocking out IP₃Rs in HEK293 cells.

Target	sgRNA Sequence (5′→3′)	Target CDs Site	Primers (5′→3′)
ITPR1	CAAAGACGACATATTAAAGG	687–706	F: TGCTGTGATTTTAGTGGCGT R: TCTCCACCCTACCCTTACCT
ITPR2	ACACGATGTCCCCTATGTAG	46–27	F: TCTTGGCCGCTGTAGTCC R: CCAGGGAAAACAAGCACACA
ITPR3	ACCCCCCTTCTCACGGAACG	1593–1574	F: GGGTTGTGGTCCAGCTTAGA R: CATCTAACCCAGTGCAAGGC