Figure 2.

Induction of HIF-1α expression by cobalt chloride (CoCl₂) or hypoxic conditions: (a) A2058, A375, and MeWo cells were treated with CoCl₂ at a concentration of 100 µM. Nuclear protein lysates were collected at 24, 48, and 72 h post-treatment. (b) Densitometric quantification of HIF-1α immunoblots relative to nuclear Lamin B1 protein expression. (c) A2058, A375, and MeWo cells were incubated on hypoxic (Hy) or normoxic (No) conditions. Nuclear protein lysates were collected at 24, 48, and 72 h post-treatment. Expression of HIF-1α was detected by Western blot; Lamin B1 was used as a loading control. (d) Densitometric quantification of HIF-1α immunoblots relative to nuclear Lamin B1 protein expression. A representative experiment is shown from three performed.