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. 2020 Feb 17;10(2):314. doi: 10.3390/biom10020314

Figure 2.

Figure 2

Acute ER stress downregulates adipsin expression in vivo. C57BL/6N mice were intraperitoneally injected with vehicle (Veh; dimethyl sulfoxide), thapsigargin (Thap; 1 mg/kg), or tunicamycin (Tun; 2.5 mg/kg). After 6 h, the mice were sacrificed. (A) Real-time RT-PCR analysis of adipsin in gWATs. (B) Real-time RT-PCR analysis of CHOP, ATF4, and GRP78 in gWATs. (C) Western blot analysis of adipsin, GRP78, and GAPDH in gWATs. GAPDH was used as a loading control. Each lane represents an individual animal. (D) Serum levels of circulating adipsin. n = 8 per group. All data are represented as the mean ± SEM. ** p < 0.01, and *** p < 0.001 vs. Veh-treated group.