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. 2020 Feb 17;10(2):314. doi: 10.3390/biom10020314

Figure 3.

Figure 3

Chronic ER stress downregulates adipsin expression in vivo. C57BL/6N mice were fed a normal chow diet (ND) or a high-fat diet (HFD) for 12 weeks. (A) Real-time RT-PCR analysis of adipsin in gWATs. (B) Real-time RT-PCR analysis of CHOP, ATF4, and GRP78 in gWATs. (C) Western blot analysis of adipsin, CHOP, ATF4, GRP78, and GAPDH in gWATs. GAPDH was used as a loading control. Each lane represents an individual animal. (D) Serum levels of circulating adipsin. n = 8 per group. All data are represented as the mean ± SEM. * p < 0.05, ** p < 0.01, and *** p < 0.001 vs. ND-fed group.