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. 2020 Feb 17;10(2):314. doi: 10.3390/biom10020314

Figure 4.

Figure 4

ER stress downregulates adipsin expression in vitro. Mature 3T3-L1 adipocytes were treated with thapsigargin (Thap; 0, 1, or 10 nM) or tunicamycin (Tun; 0, 1, or 5 μg/mL) for 0, 12, or 24 h. (A) Dose-dependent effects of Thap on adipsin mRNA at 24 h. (B) Dose-dependent effects of Tun on adipsin mRNA at 24 h. (C) Time-dependent effects of Thap on mRNA of adipsin and GRP78. (D) Time-dependent effects of Tun on mRNA of adipsin and GRP78. (E) Dose-dependent effects of Thap on protein levels of adipsin, CHOP, ATF4, GRP78, and GAPDH. GAPDH was used as a loading control. (F) Dose-dependent effects of Tun on protein levels of adipsin, CHOP, ATF4, GRP78, and GAPDH. GAPDH was used as a loading control. (G) Levels of adipsin secreted into culture media in Thap-treated cells. (H) Levels of adipsin secreted into culture media in Tun-treated cells. All data are represented as the mean ± SEM. * p < 0.05, ** p < 0.01, and *** p < 0.001 vs. non-treated cells.