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. 2020 Feb 5;9(2):365. doi: 10.3390/cells9020365

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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The effect of intracellular application of inactivated antibodies. (A–C) Inactivated TRPC4 antibody (In. Anti-TRPC4, 1:100). (A) Percentages of cells that showed persistent firing in control (5 min) and 15 min after the rupturing of the membrane. (B) Post-stimulus firing frequency (Wilcoxon test, ns, p = 0.11, n = 8). (C) Post-stimulus depolarization (paired t-test, * p ≤ 0.05, n = 8). (D–F) Inactivated TRPC5 antibody (In. Anti-TRPC5, 1:100). (D) Percentages of cells that showed persistent firing in control (5 min) and 15 min after the rupturing of the membrane. (E) Post-stimulus firing frequency (paired t-test, p = 0.82, n = 4). (F) Post-stimulus depolarization (paired t-test, p = 0.64, n = 4).