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. 2020 Jan 26;9(2):300. doi: 10.3390/cells9020300

Figure 3.

Figure 3

Association of C5aR1 expression on CD11b+ cDCs with MHC-II, CD40, CD80, CD86 and OX40L in naïve and HDM/OVA-immunized mice. (A) Costimulatory molecule expression in cDCs from either naïve WT mice (left panel) or WT mice treated once i.t. with a mixture of HDM/OVA (100 μg/40 μg) (right panel). Twenty-four hours after the in vivo treatment, the expression of MHC-II and the co-stimulatory molecules CD40, CD80, CD86, and OX40L was evaluated by measuring 10,000 events for each of the two subpopulations. (B) Comparison of MHC-II and costimulatory expression levels before and after treatment with HDM/OVA (100 μg/40 μg) in C5aR1− cDCs (left panel) and C5aR1+ cDCs (right panel). Shown is the ΔMFI of the expression levels of the examined molecules by the two subsets as mean ± SEM; the ΔMFI is defined as the mean fluorescence intensity of the signal normalized to the FMO control; n = 3–6 per group; differences between groups were evaluated by unpaired t-test, * p < 0.05; ** p < 0.01; **** p < 0.0001.