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. 2020 Feb 12;9(2):420. doi: 10.3390/cells9020420

Table 1.

List of the most used methods for the generation of organoids.

Methods for Organoid Generation
Scaffold-Based Methods
  • Micro-molding

  • Porous 3D scaffolds

  • Poly(N-isopropylacrylamide)-based thermo-responsive surfaces with controllable cell adhesion for cell sheet formation

  • Hepatocyte self-assembly on Primaria dishes

Scaffold-Free Methods
  • Hanging-drop culture

  • Cell culture on non-adhesive surface

  • Centrifugation pellet culture