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. 2020 Feb 10;12(2):411. doi: 10.3390/cancers12020411

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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E01-GS-TPD inhibits EGFR/ADAM17-dependent A431 cell proliferation. A431 cells were treated with fusion protein E01-GS-TPD at increasing concentrations for a total of 48 h. (a) confluency and (b) cell number were examined. (c) cell viability following treatment was determined by 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulphophenyl)-2H-tetrazolium (MTS). (d) cell cycle distribution was analyzed using propidium iodide. (e,f) apoptosis was detected based on membrane asymmetry to distinguish between dead (D), living (L), and apoptotic cells (A). Mean and standard deviation from (n = 3) when shown, * p < 0.05 in Tukey’s multiple comparisons test. (g) cell viability comparing boiled and non-boiled E01-GS-TPD was determined by MTS.