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Detection of IN14 by LC-MS. (A) IN14 chromatogram and electrospray ionization (ESI) (+) mass spectrum. Extracted ion chromatograms (EIC) of m/z = 236 in samples of cerebrospinal fluid (B), plasma (C), and urine (D). Mice (n = 5) were treated with IN14 for one day (red line) and five days (black line), fluid extraction and LC-MS analysis are described in the Materials and Methods section.
