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Effects of the parameters affecting ALA-induced PpIX production. Healthy donor PBMCs were activated in vitro with anti-CD3/CD28 antibodies for 3 days. (A) effects of different ALA incubation time intervals on PpIX production in resting and anti-CD3/CD28 activated CD3+ T cells; (B) the effect of cell density on ALA-induced PpIX production; (C) the effect of temperature (RT and 37 °C) on ALA-PpIX production in resting, anti-CD3/IL-2 activated and anti-CD3/CD28 activated PBMCs.
