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. 2020 Feb 12;9(2):422. doi: 10.3390/cells9020422

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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EV, EV_w/o, Prot and Prot_w/o isolates characterization by TEM, AFM and western blot. (A,B) Representative TEM and AFM images for each group of EVs or pooled protein fractions with their respective scale bars. (C,D) Typical EV markers (CD9 and CD63) identified by western blot from EV, EV_w/o samples or Prot and Prot_w/o, loading the same amount of protein for both group samples.