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. 2020 Feb 3;12(2):334. doi: 10.3390/cancers12020334

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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The sensitivities to irinotecan with modulating TOP1 and TDP1 expressions were evaluated by MTT assay. TOP1-siRNA mix, TDP1-siRNA mix, and non-targeting siRNA (160nM each) were transfected into olaparib-resistant cells or parental cells, and then treated with irinotecan for 5 d. Cell viability percentages were then measured by MTT assay, and TOP1 or TDP1 silencing was determined by western blotting (A,B). pCMV-tag2A-TOP1 or pCMV-tag2A-TDP1 and/or siTOP1 or siTDP1 were transfected into parental or olaparib-resistant cells. The sensitivity to irinotecan was calculated by MTT assay and the efficacy of transfection was confirmed by western blotting (C,D).

The sensitivities to irinotecan with modulating TOP1 and TDP1 expressions were evaluated by MTT assay. TOP1-siRNA mix, TDP1-siRNA mix, and non-targeting siRNA (160nM each) were transfected into olaparib-resistant cells or parental cells, and then treated with irinotecan for 5 d. Cell viability percentages were then measured by MTT assay, and TOP1 or TDP1 silencing was determined by western blotting (A,B). pCMV-tag2A-TOP1 or pCMV-tag2A-TDP1 and/or siTOP1 or siTDP1 were transfected into parental or olaparib-resistant cells. The sensitivity to irinotecan was calculated by MTT assay and the efficacy of transfection was confirmed by western blotting (C,D).