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. 2020 Feb 3;9(2):354. doi: 10.3390/cells9020354

Figure 1.

Figure 1

Effect of miR-28-5p re-expression in PCa cells. (A) Analysis of the miR-28-5p expression level by qRT-PCR in prostate cancer cell lines with respect to the normal cells RNA. (B) Relative expression level of miR-28-5p, evaluated by qRT-PCR, after miR-28-5p transfection in DU-145 cells. Cell migration (C,D) and invasion (E) of DU-145 cells after miR-28-5p overexpression evaluated by wound healing assay (C) and trans-well assay (D,E). (F) Relative expression of E-cadherin 1 (CDH1) and vimentin (VIM) in miR-28-5p overexpressing versus normal DU-145 cells. (G) Number of colonies formed in soft agar in DU-145 cells after miR-28-5p or CT overexpression. * p < 0.05, ** p < 0.01, *** p < 0.001, unpaired t-test.