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. 2020 Feb 4;10(2):238. doi: 10.3390/biom10020238

Table 1.

Primer sequences used for PCR.

PCR Type Genes Name Sequence (5′-3′)
Semiquantitative RT-PCR IFN-β Forward TCCAAGAAAGGACGAACATT
Reverse TGAGGACATCTCCCACGTCA
IFIT1 Forward ATGCAGTCGTAGCCTATCGC
Reverse CCTGCAAGGCCCTGTTTAGA
GAPDH Forward ACCACAGTCCATGCCATCAC
Reverse CCACCACCCTGTTGCTGTAG
qPCR IFN-β Forward AAGAGTTACACTGCCTTTGCTATC
Reverse CACTGTCTGCTGGTGGAGTTCATC
IKKε Forward CCACTTGGAGTGCAGGAAGA
Reverse CCGGAT TTCTTGTTTCGGGC
CXCL10 Forward ATCATCCCTGCGAGCCTATCC
Reverse CGGATTCAGACATCTCTGCTCATC
IFIT1 Forward ATGCAGTCGTAGCCTATCGC
Reverse CCTGCAAGGCCCTGTTTAGA
GAPDH Forward CAATGAATACGGCTACAGCA
Reverse AGGGAGATGCTCAGTGTTGG