Figure 4.

Anakinra increased apoptosis in GBM cells. T98G cells were incubated in conditioned media from direct GBM–PBMC co-cultures with or without (w/o) anakinra (Ana). (A) mRNA and protein expression of BCL-2 in T98G (n = 7, p < 0.035). One representative example of sodium dodecyl sulfate polyacrylamide gel electrophoresis is shown. (B) BAX and BCL-2 mRNA expression in T98G were quantified in qRT-PCR and the BAX/BCL-2 ratio was calculated (n = 3, p < 0.05). (C) Percentage of apoptotic T98G cells as measured by flow cytometry (n = 7, p < 0.049). * p < 0.05, ** p < 0.01.