Figure 5.

Anakinra reduced inflammatory signaling in lymphocytes co-cultured with T98G cells. T cells were isolated from PBMC after indirect co-culture with or without (w/o) anakinra (Ana). Protein levels in conditioned media from indirect co-cultures were assessed by enzyme-linked immunosorbent assay (ELISA). (A) mRNA and protein levels of proinflammatory cytokines IFNγ, IL-17, and IL-22 (mRNA: n = 13; protein: n = 10, p < 0.004). (B) mRNA expression of cytotoxic T-cell effector molecules PRF1 and GZMB (n = 9). (C) mRNA and protein level of anti-inflammatory cytokine IL-10 (mRNA: n = 13, p = 0.038; protein: n = 10, p = 0.002). * p < 0.05, ** p < 0.01.