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. 2020 Jan 27;9(2):301. doi: 10.3390/cells9020301

Figure 3.

Figure 3

Mitochondrial morphology and viability of human foreskin fibroblast (HFF-1) cells expressing the p.G362S DNM1L mutation. Human foreskin fibroblasts, transformed with a control plasmid expressing GFP (A,C) or a plasmid expressing GFP and p.G362S DNM1L mutation (B,D), were seeded in 35mm glass-bottom tissue culture plates 72 h post-transformation. The following day, the medium was replaced with fresh high-glucose medium without (A,B) additive (NA) or in the presence of bezafibrate (C,D) and incubated for 72 h. Mitochondrial morphology was visualized by MitoTracker Red CM-H2XRos (red) and was examined in cells expressing GFP (green) by fluorescence microscopy. Nuclei were stained in blue. Scale bar = 10 µM. In parallel experiments, cell viability was measured by trypan blue and the results are depicted in the histogram (E) ± SEM; * p < 0.05 compared to NA control; ^ p < 0.05 compared to the same cell with no additive.