Table 1.
Composition and nutrients content of basal diet (g kg−1).
| Ingredients | Nutrients contenta | ||
|---|---|---|---|
| Fish meal | 170.0 | Crude protein | 384.7 |
| Casein | 10.0 | Crude fat | 71.0 |
| Gelatin | 10.0 | Available phosphorus | 14.8 |
| Amino acid premixb | 170.0 | ω-3 | 15.2 |
| Leucine-glycine premixc | 120.0 | ω-6 | 13.9 |
| α-starch | 210.0 | ||
| Corn starch | 168.3 | ||
| Fish oil | 30.0 | ||
| Soybean oil | 26.0 | ||
| Vitamin premixd | 10.0 | ||
| Mineral element premixe | 20.0 | ||
| Ca(H2PO4)2 | 40.0 | ||
| Choline chloride (50%) | 10.0 | ||
| Ethoxyquin (30%) | 0.5 | ||
| Cellulose | 5.0 | ||
| XanthophyII | 0.2 |
a The values of crude protein, crude fat, and crude ash were measured. Available phosphorus, n-3 and n-6 contents calculated according to NRC (2011). b Amino acid mix (g kg−1): lysine, 125.204; histidine, 27.508; isoleucine, 39.425; arginine, 116.491; methionine, 60.071; cystine, 11.432; phenylalanine, 77.364; threonine, 83.764; tryptophan, 11.723; valine, 11.302; all ingredients were diluted with corn starch to 1 kg. c Leucine–glycine premix composition from diet 1 to 7 was as follows (g kg−1): l-leucine 0.0, 41.7, 83.3, 125.0, 166.7, 208.3, 250.0; glycine 152.8, 127.3, 101.9, 76.3, 50.9, 25.5, 0.0; corn starch 847.2, 831.0, 814.8, 798.7, 782.0, 766.2, 750.0, respectively. d Vitamin premix (g kg−1): retinyl acetate (500,000 IU g−1), 8.063; cholecalciferol (500,000 IU g−1), 0.100; dl-α-tocopherol acetate (500 g kg−1), 53.600; menadione (230 g kg−1), 0.217; cyanocobalamin (10 g kg−1), 0.100; d-biotin (20 g kg−1), 5.000; folic acid (960 g kg−1), 0.521; thiamin nitrate (900 g kg−1), 0.111; ascorbyl acetate (930 g kg−1), 86.022; niacin (990 g kg−1), 3.143; mesoinositol (990 g kg−1), 52.323; calcium-d-pantothenate (900 g kg−1), 1.667; riboflavine (800 g kg−1), 1.125; pyridoxine hydrochloride (810 g kg−1), 0.370; all ingredients were diluted with corn starch to 1 kg. e Mineral premix (g kg−1): FeSO4·H2O (300 g kg−1 Fe), 13.333; CuSO4·5H2O (250 g kg−1 Cu), 1.300; ZnSO4·H2O (345 g kg−1 Zn), 13.043; MnSO4 ·H2O (318 g kg−1 Mn), 4.717; KI (38 g kg−1 I), 1.447; NaSeO3 (10 g kg−1 Se), 1.000; MgSO4·H2O (150 g kg−1 Mg), 133.333. All ingredients were diluted with CaCO3 to 1 kg.