Table 3.
AG and ER SDEO treatment effects on Th1/Th2 cytokine secretions using primary splenocytes from female BALB/c mice.
| Th1 | Th2 | Th2/Th1 Cytokines Ratio (pg/pg) | ||
|---|---|---|---|---|
| Cytokines (pg/mL) | ||||
| Samples | Treatments (μg/mL) | IL-2 | IL-5 | IL-5/IL-2 |
| AG | control | 23.9 ± 4.5 B | 0.0 ± 0.0 B | 0.00 ± 0.00 E |
| 0.125 | 21.3 ± 3.3 B | 15.3 ± 4.9 A | 0.73 ± 0.26 C,D | |
| 0.25 | 16.8 ± 2.4 C | 13.4 ± 3.4 A | 0.82 ± 0.26 B,C | |
| 1 | 9.7 ± 2.5 D | 12.0 ± 4.0 A | 1.28 ± 0.42 A | |
| 5 | 11.1 ± 2.6 D | 11.8 ± 4.6 A | 1.13 ± 0.59 A,B | |
| LPS | 35.3 ± 5.1 A | 13.4 ± 3.1 A | 0.39 ± 0.12 D | |
| ER | control | 23.9 ± 4.5 b | 0.0 ± 0.0 d | 0.00 ± 0.00 c |
| 0.25 | 9.8 ± 1.6 c | 50.9 ± 6.1 a | 5.50 ± 1.13 b | |
| 1 | 10.3 ± 2.3 c | 49.0 ± 2.5 a,b | 4.90 ± 0.97 b | |
| 5 | 9.4 ± 2.1 c | 50.8 ± 8.3 a | 5.43 ± 1.10 b | |
| 25 | 3.0 ± 1.1 d | 45.3 ± 2.5 b | 16.8 ± 6.72 a | |
| LPS | 35.3 ± 5.1 a | 13.4 ± 3.1 c | 0.39 ± 0.12 c | |
Values are means ± SD (n = 6 biological determinations). Values within same column in the same item (AG or ER) not sharing a common superscript letter are significantly different (p < 0.05) from each other assayed using one-way ANOVA, followed by Duncan′s multiple range test. The detection sensitivity of cytokine ELISA kits used in this study was <15.6 pg/mL.