Table 4.
AG and ER SDEO treatment effects on pro- and anti-inflammatory cytokine secretions using mouse peritoneal macrophages in the absence of LPS.
| Pro-Inflammatory | Anti-Inflammatory | Pro-/Anti-Inflammatory Cytokines Secretion Ratios (pg/pg) |
|
|---|---|---|---|
| Cytokines (pg/mL) | |||
| Treatments (μg/mL) |
TNF-α | IL-10 | TNF-α/IL-10 |
| AG control | 45 ± 20 B | 78 ± 19 B | 0.63 ± 0.34 A |
| 0.125 | 26 ± 5 B | 91 ± 31 B | 0.31 ± 0.07 B |
| 0.25 | 28 ± 7 B | 84 ± 27 B | 0.35 ± 0.16 A,B |
| 1 | 33 ± 5 B | 88 ± 27 B | 0.40 ± 0.04 A,B |
| 5 | 26 ± 4 B | 102 ± 24 B | 0.26 ± 0.04 B |
| LPS | 248 ± 152 A | 735 ± 148 A | 0.33 ± 0.19 A,B |
| ER control | 45 ± 20 b | 78 ± 19 c | 0.63 ± 0.34 a |
| 0.25 | 26 ± 18 b | 108 ± 14 c | 0.25 ± 0.20 b |
| 1 | 25 ± 14 b | 104 ± 9 c | 0.24 ± 0.13 b |
| 5 | 23 ± 11 b | 120 ± 7 c | 0.19 ± 0.08 b |
| 25 | 28 ± 14 b | 339 ± 34 b | 0.08 ± 0.04 b |
| LPS | 248 ± 152 a | 735 ± 148 a | 0.33 ± 0.19 b |
Values are means ± SD (n = 4 biological determinations). Values within same column in the same item (AG or ER) not sharing a common superscript letter are significantly different (p < 0.05) from each other assayed using one-way ANOVA, followed by Duncan′s multiple range test. The detection sensitivity of cytokine ELISA kits used in this study was <15.6 pg/mL.