Table 5.
AG and ER SDEO treatment effects on pro- and anti-inflammatory cytokine secretions using LPS-stimulated peritoneal macrophages from female BALB/c mice.
| Pro-Inflammatory | Anti-Inflammatory | Pro-/Anti-Inflammatory Cytokine Secretion Ratios (pg/pg) |
|
|---|---|---|---|
| Cytokines (pg/mL) | |||
| Treatments (μg/mL) |
TNF-α | IL-10 | TNF-α/IL-10 |
| VC | 144 ± 30 C | 190 ± 63 B | 0.81 ± 0.26 A |
| control (LPS alone) |
499 ± 81 B | 1005 ± 282 A | 0.52 ± 0.14 B |
| AG 0.125 | 592 ± 53 A | 1292 ± 405A | 0.50 ± 0.16 B |
| 0.25 | 580 ± 74 A,B | 1274 ± 371 A | 0.48 ± 0.10 B |
| 1 | 546 ± 68 A,B | 1234 ± 389 A | 0.47 ± 0.14 B |
| 5 | 557 ± 69 A,B | 1308 ± 405 A | 0.46 ± 0.15 B |
| VC | 144 ± 30 d | 190 ± 63 c | 0.81 ± 0.26 a |
| control (LPS alone) |
499 ± 81 a | 1005 ± 282 a | 0.52 ± 0.14 b |
| ER 0.25 | 239 ± 39 c | 726 ± 193 b | 0.34 ± 0.05 c |
| 1 | 222 ± 39 c | 712 ± 120 b | 0.31 ± 0.02 c |
| 5 | 235 ± 49 c | 817 ± 149 a,b | 0.29 ± 0.02 c |
| 25 | 396 ± 47 b | 922 ± 146 a,b | 0.43 ± 0.03 b,c |
Values are means ± SD (n = 6 biological determinations). Values within same column in the same item (AG or ER) not sharing a common superscript letter are significantly different (p < 0.05) from each other assayed using one-way ANOVA, followed by Duncan′s multiple range test. The sensitivity of cytokine ELISA kits used in this study was <15.6 pg/mL.