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. 2020 Feb 7;9(2):380. doi: 10.3390/cells9020380

Figure 8.

Figure 8

Increased autophagy upon acute PARP2 silencing. PARP2 was transiently silenced in C2C12 cells using siRNA targeting PARP2 (n = 3). Cells were transfected with siRNA for 48 h, treated with 1 mM AICAR, 500 µM nicotinamide-riboside (NR), and 25 µM EX-527 for 24 h, then LC3 expression was assessed by confocal microscopy. Alexa Fluor 488-linked LC3 specific antibody was used and the nuclei were visualized using DAPI and vesicles were counted. #, ## and ### represent statistically significant differences between the control and treated cells at p < 0.05, p < 0.01 or p < 0.001, respectively. *, **, and *** represent statistically significant differences between the scPARP2 and shPARP2 cells at p < 0.05, p < 0.01, and p < 0.001, respectively. For the determination of statistical significance, ANOVA test was used followed by Tukey’s post hoc test.