Figure 5.

Claramine inhibits cell adhesion, migration, invasion and proliferation triggered by IL-13. (A) U87MG, KM12SM and A2780 cells, treated with or without Claramine, were exposed to IL-13, lysed and the extracts analyzed by Western blot to detect pSrc Y₅₃₀ and total Src. (B) KM12SM cells were subjected to cell adhesion. (C) KM12SM and U87MG cells were subjected to MTT assays. The three cell lines were subjected to (D) migration and (E) invasion assays. All the experiments were done in the presence or absence of IL-13, Claramine and/or anti-IL13Rα2 blocking antibodies. Cell adhesion/migration/invasion and proliferation were significantly increased by addition of IL-13 (** p < 0.01; *** p < 0.001) and inhibited by treatment with Claramine or the blocking antibody (◊◊ p < 0.01; ◊◊◊ p < 0.001).