Figure 3.

Kinetic profiles of E1 metabolite formation in platinum-sensitive and -resistant HGSOC cells. The kinetics of (A–B) E2 formation, (C–D) E1 sulfation, (E–F) E2 sulfation, and (G–H) E2 glucuronidation were calculated following the incubation of all HGSOC cell lines with 0 to 2000 nM E1 as a hormone precursor for 48 h. Data are displayed as Michaelis–Menten and Lineweaver–Burk plots and represent the means ± SD of three independent experiments. Green curves indicate sensitivity and red curves indicate resistance against carboplatin to the investigated HGSOC cell lines. Differences were statistically significant between these two groups (p < 0.05).